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Literature summary extracted from

  • Kadonosono, T.; Chatani, E.; Hayashi, R.; Moriyama, H.; Ueki, T.
    Minimization of cavity size ensures protein stability and folding: structures of Phe46-replaced bovine pancreatic RNase A (2003), Biochemistry, 42, 10651-10658.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression in Escherichia coli Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.18 hanging drop vapor diffusion method Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 F46A mutant with adversely affected conformational stability and folding speed Bos taurus
4.6.1.18 F46L mutant with adversely affected conformational stability and folding speed Bos taurus
4.6.1.18 F46V mutant with adversely affected conformational stability and folding speed Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.18 0.41
-
 cytidine-2',3'-cyclic monophosphate F46A mutant, pH 5.5, 25ºC Bos taurus
4.6.1.18 0.42
-
 cytidine-2',3'-cyclic monophosphate F46L mutant, pH 5.5, 25ºC Bos taurus
4.6.1.18 0.51
-
 cytidine-2',3'-cyclic monophosphate wild type enzyme, pH 5.5, 25ºC Bos taurus
4.6.1.18 0.64
-
 cytidine-2',3'-cyclic monophosphate F46V mutant, pH 5.5, 25ºC Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus P61823
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 cytidine-2',3'-cyclic monophosphate + H2O hydrolysis reaction Bos taurus 3'-CMP
-
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